RIPK3 Expression in Fibroblasts in an in vivo and in vitro Skin Wound Model: A Controversial Result

Izumov, I.S.; Shitova, M.S.; Sabirov, M.S.; Sheleg, S.A.; Cherkashina, O.L.; Kalabusheva, E.P.; Vorotelyak, E.A.; Morgun, E.I.

Авторы: Izumov I.S., Shitova M.S., Sabirov M.S., Sheleg S.A., Cherkashina O.L., Kalabusheva E.P., Vorotelyak E.A., Morgun E.I.
Журнал: Acta Naturae (англоязычная версия)
Том: 15
Номер: 4
Год издания: 2023
Издательство: Park Media Ltd
Местоположение издательства: Moscow, Russia (Federation)
Первая страница: 65
Последняя страница: 74
DOI: 10.32607/actanaturae.25452
Аннотация: One of the major problems of regenerative medicine is the development of hypertrophic scars andkeloids. The protein kinase RIPK3 is involved in necroptosis; however, recent evidence indicates that it alsohas non-canonical functions, including its involvement in the development of renal fibrosis. The aim of ourwork was to study the expression of RIPK3 in mouse and human skin models of fibrotic processes. A sub-population of RIPK3+Vim+ cells was found in both human keloid and a mouse wound, with the cell num-ber being significantly greater in the mouse wound bed compared to healthy skin. Real-time polymerasechain reaction (RT-PCR) detected expression of the Ripk3 and fibroblast biomarkers Acta2, Fap, Col1a1, andFn1 in the cells isolated from the wound bed, indicating that RIPK3 can be expressed by wound bed fibro-blasts. An analysis of the human fibroblasts stained with anti-RIPK3 antibodies demonstrated an increase inthe fluorescence intensity in the presence of lipopolysaccharide (LPS) at concentrations of 5, 10, 25, 50, and100 ng/ml and TGF-β at concentrations of 0.1, 1, 2, and 5 ng/ml compared to the control. At the same time,the expression levels of RIPK3 and fibroblast activation markers in the presence of TGF-β and LPS did notdiffer significantly from the control. It is possible that RIPK3 expression in wound fibroblasts is not direct-ly associated with fibrotic processes, and that kinase plays a different, yet unknown role in wound healing.
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RIPK3 Expression in Fibroblasts in an in vivo and in vitro Skin Wound Model: A Controversial Resultстатья

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