Microphase Mechanism of “Superquenching” of Luminescent Probes in Aqueous Solutions of DNA and Some Other Polyelectrolytesстатья
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Дата последнего поиска статьи во внешних источниках: 2 июня 2014 г.
Аннотация:A new approach in terms of microphase model of aqueous solutions of
polyelectrolytes is proposed for explanation of a very strong quenching of luminescent
probes (“superquenching”) in these solutions. This phenomenon is used in literature for
creation of extremely sensitive chemical and biosensors and was attributed predominantly
to efficient energy or electron transfer. Microphase approach considers this phenomenon in
terms of local concentrations of both the luminescent compound and of the quencher in
microphase, formed by DNA and other polyelectrolytes, which can be several (4−10)
orders of magnitude greater than their apparent concentrations in solution. Large local
concentrations of the light absorbing centers in the microphase also provide conditions for
aggregation of these centers and efficient energy transfer, which provides a significant increase in quenching constants (∼102−105). Microphase approach provides good quantitative description of all the features of the superquenching, new possibilities for
analysis and control of kinetics of DNA reactions, and for improvement of the sensitivity of luminescent sensors. It revealsnonspecific localization of the luminescent centers and of Aun nanoparticles in different positions of DNA molecules that hinders
from the simultaneous use of optical methods and electron or tunneling microscopy for the combined study of the structure of DNA.