Immunotechniques for the Group Determination of Macrolide Antibiotics Traces in the Environment Using a Volume-Mediated Sensitivity Enhancement Strategy

Burkin, M.A.; Tevyashova, A.N.; Bychkova, E.N.; Melekhin, A.O.; Galvidis, I.A.

Авторы: Burkin Maksim A., Tevyashova Anna N., Bychkova Elena N., Melekhin Artem O., Galvidis Inna A.
Журнал: Biosensors
Том: 13
Номер: 10
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 921
Последняя страница: 921
Номер статьи: 921
DOI: 10.3390/bios13100921
Аннотация: Macrolide antibiotics, which are effective antimicrobial agents, are intensively used inhuman and veterinary medicine, as well as in agriculture. Consequently, they are found all overthe world as environmental pollutants, causing harm to sensitive ecological communities and provoking a selection of resistant forms. A novel azithromycin derivative, which was used as haptenconjugate, ensured the group immunorecognition of six major macrolide representatives (105–41%),namely erythromycin, erythromycin ethylsuccinate, clarithromycin, roxithromycin, azithromycin,and dirithromycin in a competitive immunoassay based on anti-clarithromycin antibodies. Theheterologous hapten-based ELISA format resulted in a 5-fold increase in sensitivity, with an IC50value of 0.04 ng/mL for erythromycin. In this study, we proposed an underexploited strategyin an immunoassay field to significantly improve the detectability of analytes in environmentalsamples. Unlike most approaches, it does not require special enhancers/amplifiers or additionalconcentration/extraction procedures; instead, it involves analyzing a larger volume of test samples.A gradual volume increase in the samples (from 0.025 to 10 mL) analyzed using a direct competitiveELISA, immunobeads, and immunofiltration assay formats based on the same reagents resulted ina significant improvement (more than 50-fold) in assay sensitivity and detection limit up to 5 and1 pg/mL, respectively. The suitability of the test for detecting the macrolide contamination of naturalwater was confirmed by the recovery of macrolides from spiked blank samples (71.7–141.3%). During2022–2023, a series of natural water samples from Lake Onega and its influents near Petrozavodskwere analyzed, using both the developed immunoassay and HPLC-MS/MS. The results revealed nocontamination of macrolide antibiotic.
Добавил в систему: Мелехин Артем Олегович

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Immunotechniques for the Group Determination of Macrolide Antibiotics Traces in the Environment Using a Volume-Mediated Sensitivity Enhancement Strategyстатья

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