Effects of Addition of Blocking Agents on Fluorescence Polarization Immunoassay of Okadaic Acid Using a Microfluidic Device

Авторы: CHIDA Shunsuke, TAKAHASHI Kazuki, FUKUYAMA Mao, KASUYA Motohiro, MAEKI Masatoshi, ISHIDA Akihiko, TANI Hirofumi, SHIGEMURA Koji, ZHERDEV Anatoly V., EREMIN Sergei A., HIBARA Akihide, TOKESHI Manabu
Журнал: Bunseki Kagaku
Том: 72
Номер: 3
Год издания: 2023
Издательство: Japan Society for Analytical Chemistry
Местоположение издательства: Japan
Первая страница: 133
Последняя страница: 138
DOI: 10.2116/bunsekikagaku.72.133
Аннотация: In analysis using microfluidic devices, adsorption of samples and other substances on the channel walls can be a problem. Especially in immunoassays using polydimethylsiloxane (PDMS) devices, the adsorption of antibodies and other substances to be measured greatly affects measurement performance. In this study, we investigated the effect of the addition of two blocking agents on the detection sensitivity of okadaic acid (OA), a diarrheal shellfish poison, by fluorescence polarization immunoassay. In addition, the dissociation constants for the antibody and tracer were calculated from the obtained calibration curve.
Добавил в систему: Еремин Сергей Александрович

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