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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Cas9‐targeted Nanopore sequencing rapidly elucidates the transposition preferences and DNA methylation profiles of mobile elements in plantsстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Merkulov Pavel, Gvaramiya Sofya, Dudnikov Maxim, Komakhin Roman, Omarov Murad, Kocheshkova Alina, Konstantinov Zakhar, Soloviev Alexander, Karlov Gennady, Divashuk Mikhail, Kirov Ilya
- Журнал: Journal of Integrative Plant Biology
- Год издания: 2023
- Издательство: Blackwell Publishing Inc.
- Местоположение издательства: United Kingdom
- DOI: 10.1111/jipb.13555
- Аннотация: Transposable element insertions (TEIs) are an important source of genomic innovation by contributing to plant adaptation, speciation, and the production of new varieties. The often large, complex plant genomes make identifying TEIs from short reads difficult and expensive. Moreover, rare somatic insertions that reflect mobilome dynamics are difficult to track using short reads. To address these challenges, we combined Cas9-targeted Nanopore sequencing (CANS) with the novel pipeline NanoCasTE to trace both genetically inherited and somatic TEIs in plants. We performed CANS of the EVADÉ (EVD) retrotransposon in wild-type Arabidopsis thaliana and rapidly obtained up to 40x sequence coverage. Analysis of hemizygous T-DNA insertion sites and genetically inherited insertions of the EVD transposon in the ddm1 genome uncovered the crucial role of DNA methylation in shaping EVD insertion preference. We also investigated somatic transposition events of the ONSEN transposon family, finding that genes that are downregulated during heat stress are preferentially targeted by ONSENs. Finally, we detected hypomethylation of novel somatic insertions for two ONSENs. CANS and NanoCasTE are effective tools for detecting TEIs and exploring mobilome organization in plants in response to stress and in different genetic backgrounds, as well as screening T-DNA insertion mutants and transgenic plants.
- Добавил в систему: Меркулов Павел Юрьевич