Morphogenesis of osteoid structures during cultivation of mesenchemic stromal cells on fibrillary collagen in the presence of silicoalumophosphateстатья
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Дата последнего поиска статьи во внешних источниках: 20 февраля 2024 г.
Аннотация:The effect of the Biosit-Sr Elkor glass-crystalline material on the early stages of morphogenesis ofosteoid structures in cultures of mesenchymal stromal cells of bone marrow was studied. The maximum cultivation time was 28 days. Morphological investigations were performed with scanning electron microscopy. It was found that silicoaluminophosphate facilitated the generation of osteoid structures; enhanced the synthesis, compaction, and mineralization of fibrillar collagen; and activated matrix vesicles by hyperplasia, hypertrophy, and excretion. During cultivation, glass-crystalline material partially dissolved and disintegrated. Chemical substances with cross-linking, surface-active, and mineralizing properties came out into the nutrient medium. By means of surface-active agents, compacted collagen fibers were straightened and perforated, forming diversified lamellar structures, the precursors of bone plates. The mineralizing effect is mediated by increased homo- and heterogeneous nucleation of calcium phosphates and deposition of mineral concretions in differentiating cells and fibers of the collagen substrate, which accelerates the involution of cellculture. It was concluded that