Rapid Method for the Detection of Metabolite of Lewisite – 2-Chlorovinylarsonous Acid in Urine by Liquid Chromatography-Negative Electrospray-Tandem Mass Spectrometryтезисы доклада
Дата последнего поиска статьи во внешних источниках: 17 октября 2017 г.
Аннотация:The development, production, stockpiling and use of chemical weapons are prohibited
under the Chemical Weapons Convention. In cases of alleged use of chemical warfare
(CW) agents, environmental samples may be collected and analyzed for agents and their
degradation products presented as a supporting evidence of a CW attack. Biomedical
samples, e.g. blood and urine, may be analyzed for biological markers of poisoning as
evidence that individuals have been exposed to a CW agent. Biomedical sample analysis
also has applications in exposure monitoring, e.g. in individuals engaged in demilitarization
activities, and for the diagnosis of poisoning prior to the administration of medical
countermeasures.
Lewisite [dichloro(2-chlorovinyl)arsine], a highly toxic chemical warfare agent with
vesicant properties, was developed during World War I. Lewisite irritates the skin and eyes
and is also poisonous when inhaled; but its clinical effects appear within seconds of exposure.
Lewisite is very reactive; in aqueous media, it rapidly hydrolyzes to a stable water
soluble derivative, 2-chlorovinylarsonous acid (CVAA), which is also toxic.
Several analytical methods have measured CVAA to determine lewisite exposure, etc.
the authors determined CVAA in urine by using GC-MS for the 1,3-propanedithiol
derivative. Several methods, based on the detection of CVAA, have been reported for the
identification of lewisite in environmental and biological samples. These approaches
have advantages but may require derivatization that add to sample preparation time.
The main purpose of our work was to develop a rapid, sensitive technique for CVAA
determination in urine samples.
We present a method for the detection of CVAA in human and rat urines spiked with
CVAA that is based on the materials used by liquid chromatography interfaced to negative
ion-electrospray ionization-tandem mass spectrometry. Columns under gradient conditions
were used. By automating and optimizing several sample cleanup steps, the method
provides the LC-MS/MS instrument with sufficiently clean concentrated samples, at a rate
comparable to that at which it quantifies them. The use of fast, automated sample preparation
steps, employing separate solid-phase extraction analyte isolation, provides samples that are sufficiently clean and the 1 ng/ml limit of detection to be achieved. Thus, the
method is well suited for the purpose of the biomonitoring of lewisite exposure in the
event of a mass-casualty terrorist incident or in case of an accident at CW storage units
characterized by high sample loads and the low concentrations to be detected.