The Role of Osmolytes and Membrane Lipids in the Adaptation of Acidophilic Fungiстатья
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Дата последнего поиска статьи во внешних источниках: 20 февраля 2024 г.
Аннотация:cidophiles maintain near-neutral intracellular pH using proton pumps. We have sug-gested the protective role of osmolytes and membrane lipids in the adaptation to an acidic envi-ronment. Previously we have observed, for the first time, high levels of trehalose in acidophilicbasidiomycete Sistotrema brinkmannii. Here, we have studied the composition of both osmolytesand membrane lipids of two more acidophilic fungi. Trehalose and polyols were among the mainosmolytes during growth under optimal conditions (pH 4.0) in basidiomycete Phlebiopsis giganteaand ascomycete Mollisia sp. Phosphatidic acids, phosphatidylethanolamines, phosphatidylcholines,and sterols, were predominant membrane lipids in both fungi. P. gigantea had a narrow optimum ofgrowth at pH 4.0, resulting in a sharp decline of growth rate at pH 2.6 and 5.0, accompanied by adecrease in the number of osmolytes and significant changes in the composition of membrane lipids.In contrast, Mollisia sp. had a broad optimal growth range (pH 3.0–5.0), and the number of osmolyteseither stayed the same (at pH 6.0) or increased (at pH 2.6), while membrane lipids compositionremained unchanged. Thus, the data obtained indicate the participation of osmolytes and membranelipids in the adaptation of acidophilic fungi.