Comparison of Novel HILIC Stationary Phases with Different Functional Layer Structures

Chernobrovkina, A.V.; Chikurova, N.Y.; Shemiakina, A.O.; Shpigun, O.A.

Авторы: Chernobrovkina A.V., Chikurova N.Y., Shemiakina A.O., Shpigun O.A.
Сборник: HPLC 2023
Тезисы
Год издания: 2023
Аннотация: Selectivity of stationary phases for hydrophilic interaction liquid chromatography (HILIC) iscrucial for choosing a column for separating target polar compounds. It is important to know theeffect of each functional group on the chromatographic properties of the material. Establishingthe trends in selectivity changes with variations of adsorbent structure at different stages ofpreparation is useful for regulating the interactions of the stationary phase with analytes andcreating novel materials with improved performance.This work compares a set of home-made silica-based stationary phases for HILIC with varioustypes of coatings, including monomeric amide ligands, linear polymers such as poly(ethyleneglycol) (PEG) and polyethyleneimine, glycopeptide antibiotic macromolecules, and zwitterions.Functional groups were attached to the substrate either directly or using 1,4-butanedioldiglycidyl ether as a linker. Synthesized adsorbents were characterized andcompared using the Tanaka test for hydrophilic stationary phases. Their chromatographicperformance was evaluated using model mixtures of neutral, zwitterionic, and negatively andpositively charged polar analytes.Discussion of the modification pathways leading to better selectivity and efficiency toward eachclass of analytes is presented. The role of the linker used for attaching different structure unitsis also demonstrated in the work. Evaluating electrostatic interactions impact on HILIC retentionfor polar charged analytes provided a possibility for choosing the column with reasonableretention and high separation selectivity for both positively and negatively charged compounds.The prepared columns were compared to commercially available ones in terms of theirhydrophilic retention and partitioning properties. Introducing highly hydrophilic amide groups orpolar polymers provided the highest hydrophilic partitioning properties, while polymer-coatedphases demonstrated improved hydrophilic retention among the home-made and commerciallyavailable columns. PEG-coated phases demonstrated the highest retention factors for sugarsand amino acids but required their longer separation. Creating amide functionality resulted inthe highest efficiency for all the tested classes of compounds (up to 50 000 N/m) and the bestseparation of either sugars or water-soluble vitamins.The obtained results provided better understanding of the factors affecting stationary phaseselectivity and allowed to reveal the columns with improved performance for certain class ofanalytes. It was also shown that changing conditions and including analytes providing specificinteractions with particular surfaces allowed to complement column characterization andsimplify the prediction of retention and selectivity parameters.
Добавил в систему: Чикурова Наталья Юрьевна

	ИСТИНА	Войти в систему Регистрация
	Интеллектуальная Система Тематического Исследования НАукометрических данных
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

Интеллектуальная Система Тематического Исследования НАукометрических данных

Comparison of Novel HILIC Stationary Phases with Different Functional Layer Structuresтезисы доклада

Прикрепленные файлы