Activation of NF-kappa B transcription factor in human neutrophils by sulphatides and L-selectin cross-linkingстатья

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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.

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[1] Activation of nf-kappa b transcription factor in human neutrophils by sulphatides and l-selectin cross-linking / D. V. Turutin, E. A. Kubareva, M. A. Pushkareva et al. // FEBS Letters. — 2003. — Vol. 536, no. 1-3. — P. 241–245. Sulphated galactocerebroside (sulphatide) has been established as a ligand for L-selectin and shown to trigger intracellular signals in human neutrophils. We have found that sulphatide activated transcription factor NF-kappa B in human neutrophils in a concentration-dependent manner whereas non-sulphated galactocerebroside did not demonstrate such an effect. The activation was inhibitable by pretreatment with primary monoclonal anti-L-selectin antibody (clone LAM1-116). Binding of the primary antibody to L-selectin was insufficient to induce NF-kappa B activation but cross-linking of L-selectin with a secondary antibody was effective. alpha-Chymotrypsin, the agent known to shed L-selectin, activated NF-kappa B by itself. The response to sulphatides was inhibited by jasplakinolide, an actin-polymerising agent known to downregulate surface expression of L-selectin, Fc gamma RIIIb, CD43 and CD44. Recently we have reported that sulphatide stimulated the attachment of human neutrophils to collagen via Mac1 (CD11b/CD18) integrin [Sud’ina et al., Biochem. J. 359 (2001) 621-629]. We now show signalling from sulphatide to NF-kappa B activation and discuss its involvement in neutrophil adhesion. [ DOI ]

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