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New method of in vitro culturing of pigment retinal epithelium in the structure of the posterior eye sector of adult ratстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Grigoryan E.N., Novikova Yu P., Kilina O.V., Philippov P.P.
- Журнал: Bulletin of Experimental Biology and Medicine
- Том: 144
- Номер: 4
- Год издания: 2007
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 618
- Последняя страница: 625
- DOI: 10.1007/s10517-007-0389-z
- Аннотация: We propose a new method of organotypic roller 3D-culturing of the posterior sector of the eye. The method allows maintaining tissue viability in vitro for 14 days (which considerably surpasses the capacities of stationary culturing) and studying of the behavior of pigment retinal epithelial cells and choriocapillary membrane. Using this method we demonstrated phenotypic transformation, migration, and proliferation of pigment retinal epithelial cells under conditions of roller organotypic culture. In the absence of the retina, these cells exhibit properties of scavenger cells (phagocytes) both within and outside the layer. Under conditions of roller culturing in vitro, cells of the pigment retinal epithelium undergo changes similar to those observed in various retinal pathologies in vivo, including age-associated changes. Here we discuss the possibility of using the proposed method for evaluation of the effect of various factors added to the culture medium on the pigment epithelium, for modeling of processes developing in damaged pigment epithelium or under conditions of various pathologies, and for the study of regeneration responses in cells of pigment retinal epithelium in adult vertebrates.
- Добавил в систему: Филиппов Павел Павлович