A Robust Homogeneous Fluorescence Polarization Immunoassay for Rapid Determination of Erythromycin in Milk

Duan, C.; Zhang, H.; Zhang, Y.; Li Qiang; Li Peipei; Mari, G.M.; Eremin, S.A.; Shen, J.; Wang, Z.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 1 мая 2024 г.

Авторы: Duan Changfei, Zhang Huiyan, Zhang Yingjie, Li Qiang, Li Peipei, Mari Ghulam Mujtaba, Eremin Sergei A., Shen Jianzhong, Wang Zhanhui
Журнал: FOODS
Том: 12
Номер: 8
Год издания: 2023
Первая страница: 1581
DOI: 10.3390/foods12081581
Аннотация: Erythromycin (ERY) is one of the most common macrolides applied in veterinary medicine to treat diseases or as a feed additive for animal growth promotion. Long-term irrational use of ERY could lead to residues in animal-derived food and the emergence of drug-resistant strains, posing potential threats to human health. In this study, a highly sensitive, specific, robust, and rapid fluorescence polarization immunoassay (FPIA) for the determination of ERY in milk has been described. Herein, to achieve high sensitivity, five tracers of ERY with different fluorescein structures were synthesized and paired with three monoclonal antibodies (mAbs). Under the optimized conditions, the combination of mAb 5B2 and tracer ERM-FITC achieved the lowest IC50 value in the FPIA with 7.39 μg/L for ERM. The established FPIA was used to detect ERY in milk, revealing a limit of detection (LOD) of 14.08 μg/L with recoveries of 96.08–107.77% and coefficients of variations (CVs) of 3.41–10.97%. The total detection time of the developed FPIA was less than 5 min from the addition of samples to the result readout. All the above results showed that the proposed FPIA in this study was a rapid, accurate, and simple method for the screening of ERY in milk samples.
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A Robust Homogeneous Fluorescence Polarization Immunoassay for Rapid Determination of Erythromycin in Milkстатья

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