Biocontrol Potential of Novel Borrelidin-Producing Streptomyces rochei 3IZ-6 from Soil

Shirokikh, I.G.; Osterman, I.A.; Lukianov, D.A.; Marina, V.I.; Biryukov, M.V.; Belozerova, O.A.; Guglya, E.B.; Shirokikh, A.A.; Nazarova, Y.I.; Bokov, N.A.; Zakalyukina, Y.V.

Авторы: Shirokikh I.G., Osterman I.A., Lukianov D.A., Marina V.I., Biryukov M.V., Belozerova O.A., Guglya E.B., Shirokikh A.A., Nazarova Ya I., Bokov N.A., Zakalyukina Yu V.
Журнал: Eurasian Soil Science
Том: 56
Номер: 5
Год издания: 2023
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 619
Последняя страница: 627
Оригинальные версии: Новый продуцент боррелидина Streptomyces rochei 3iz-6 и его перспективы в защите растений
DOI: 10.1134/S1064229323600161
Аннотация: A complex of soil actinomycetes in the upper horizon of Grumusol (Vertisol) on the western coast of Lake Kinneret in the vicinity of Tiberias (Lower Galilee, Israel) was studied. A high-throughput screening of the antibiotic activity of 26 isolates of the genus Streptomyces using on a dual reporter system revealed the strain 3IZ-6, which had the ability to inhibit protein synthesis. This strain was assigned to Streptomyces rochei according to the polyphase taxonomy approach. The active substance of S. rochei 3IZ-6 was isolated and purified using solid-phase extraction and high-performance liquid chromatography. Toeprint analysis and mass spectrophotometric data allowed us to identify this active compound as borrelidin, a known inhibitor of protein biosynthesis. When co-cultivated on solid media, strain 3IZ-6 is able to suppress the growth of phytopathogenic microorganisms Fusarium solani, F. sambucinum, and Botrytis cinerea, as well as the quarantineobject Curtobacterium flaccumfaciens. The cultural liquid of 3IZ-6 had a pronounced phytotoxic effect on the seeds of common wheat (Triticum aestivum L.). Strain Streptomyces rochei 3IZ-6 can find application in biotechnology as a producer of borrelidin, a valuable natural compound with a wide spectrum of antibiotic activity, which can be used as a biocontrol agent for crop protection from phytopathogens and weeds.
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