Enzyme engineering for the development of bionanomaterials for medical applicationsтезисы доклада

Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.

Работа с тезисами доклада


[1] Enzyme engineering for the development of bionanomaterials for medical applications / N. L. Klyachko, E. A. Zaitseva, E. N. Efremenko et al. // Abstracts of International Conference "Biocatalysis-2013: Fundamentals & Applications". — Moscow, 2013. — P. 55–55. In 2010, the Laboratory of Chemical Design of Bionanomaterials was established at the MV Lomonosov Moscow State University. The main activities of the laboratory was directed to fundamentals and applications of new classes of bionanosystems based on proteins, enzymes, polymer coatings, magnetic nanoparticles for treatment and diagnostics of a number of diseases such as cardiovascular, brain CNS diseases and cancer at al. Special importance was attached to the development of the approach based on the technology of NanoZYMES™ for therapeutic proteins immobilization and functions (USP WO №2008/141155 A1). Using this approach novel types of nanozymes have been obtained. So, organophosphate hydrolase containing nanozymes possessing the ability to decompose organophosphorus neurotoxins were synthesized as well as antioxidative nanozymes based on superoxide dismutase for eye inflammation and spinal marrow injury treatment. Nanozymes containing stabilized bacteriolytic enzymes PlyC and LysK are promising for the treatment as antibacterials. Hybrid nanomaterials containing enzymes and superparamagnetic nanoparticles promising for theranostics were created. Inter alia, magnetic iron oxide nanoparticles stabilized by BSA and conjugated with monoclonal antibodies to vascular endothelial growth factor have been synthesized. The potential for diagnosing brain tumors via the vector MRI-contrast agent based on superparamagnetic nanoparticles in diagnostics of brain tumors was shown. The procedure of preparative synthesis of nanoparticles with a magnetic core and a gold shell, modified by organic ligands and enzymes, was designed. The magnetic materials obtained, have been studied in conditions of a low-frequency magnetic field using a device specially constructed for the purpose. Effects of low frequency magnetic fields on the conformation and the catalytic activity of enzymes attached to the surface of magnetic nanoparticles, which is the first example of a mechanical-magnetic effect in nanobiocatalysis, have been discovered. These studies offer the possibility of superparamagnetic materials as a relay for remote monitoring of chemical reactions, which can be used in the construction of a number of functional units, including the controlled release of drugs.

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