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Fragmentation of human cardiac troponin T after acute myocardial infarctionстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Katrukha Ivan A., Riabkova Natalia S., Kogan Alexander E., Vylegzhanina Alexandra V., Mukharyamova Kadriya Sh, Bogomolova Agnessa P., Zabolotskii Artur I., Koshkina Ekaterina V., Bereznikova Anastasia V., Katrukha Alexey G.
- Журнал: Clinica Chimica Acta
- Том: 542
- Год издания: 2023
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 117281
- DOI: 10.1016/j.cca.2023.117281
- Аннотация: BackgroundBlood measurement of cardiac troponin T (cTnT) is one of the most widespread methods of acute myocardial infarction (MI) diagnosis. cTnT degradation may have a significant influence on the precision of cTnT immunodetection; however, there are no consistent data describing the level and sites of cTnT proteolysis in the blood of MI patients. In this study, we bordered major cTnT fragments and quantified their relative abundance in the blood at different times after MI.MethodsSerial heparin plasma samples were collected from 37 MI patients 2–37 h following the onset of MI. cTnT and its fragments were studied by western blotting and immunofluorescence analysis using monoclonal antibodies specific to various cTnT epitopes.ResultscTnT was present in the blood of MI patients as 23 proteolytic fragments with an apparent molecular mass of ∼ 8–37 kDa. Two major sites of cTnT degradation were identified: between amino acid residues (aar) 68 and 69 and between aar 189 and 223.Analysis of the abundance of cTnT fragments showed an increase in the fraction of free central fragments in the first few hours after MI, while the fraction of the C-terminal fragments of cTnT remained almost unchanged.ConclusioncTnT progressively degrades after MI and appears in the blood as a mixture of 23 proteolytic fragments. The cTnT region approximately bordered by aar 69–158 is a promising target for antibodies used for measurement of total cTnT.
- Добавил в систему: Катруха Иван Алексеевич