Synthesis of Cy5-Labelled C5-Alkynyl-modified cytidine triphosphates via Sonogashira coupling for DNA labellingстатья
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Дата последнего поиска статьи во внешних источниках: 17 июля 2024 г.
Аннотация:New applications of palladium-catalyzed Sonogashira-type cross-coupling reaction between C5-halogenated 2′-deoxycytidine-5′-monophosphate and novel cyanine dyes with a terminal alkyne group have been developed. The present methodology allows to synthesize of fluorescently labeled C5-nucleoside triphosphates with different acetylene linkers between the fluorophore and pyrimidine base in good to excellent yields under mild reaction conditions. Modified 2′-deoxycytidine-5′-triphosphates were shown to be good substrates for DNA polymerases and were incorporated into the DNA by polymerase chain reaction.