Enzyme-Linked Immunosorbent Assay of Autoantibodies against the Human β1-Adrenergic Receptor Using Recombinant Antigens

Авторы: Grigorenko V.G., Andreeva I.P., Melnichuk E.A., Levashov P.A.
Журнал: Moscow University Chemistry Bulletin
Том: 78
Номер: 2
Год издания: 2023
Издательство: Allerton Press Inc.
Местоположение издательства: United States
Первая страница: 95
Последняя страница: 101
DOI: 10.3103/S0027131423020025
Аннотация: E. coli strains are created as producers of recombinant β1-adrenoreceptor epitopes as part of chimeric proteins. The corresponding epitope sequences are located in the C-terminal region of the human heart fatty acid binding protein (hH-FABP) and separated from it by the linker sequence (Gly4Ser)3. A solid-phase enzyme-linked immunosorbent assay (ELISA) to detect autoantibodies against the β1-adrenergic receptor in human blood serum based on a recombinant epitope is developed. Blood sera of patients (N = 76) with various diagnoses of cardiopathologies and other diseases are analyzed. A significantly high level of autoantibodies to the β1-adrenergic receptor is detected in some patients with a confirmed diagnosis of cardiovascular diseases, in most cases those with a diagnosis of acute myocardial infarction.
Добавил в систему: Григоренко Виталий Георгиевич

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