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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Differential Impact of Hexuronate Regulators ExuR and UxuR on the Escherichia coli Proteomeстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Bessonova Tatiana A., Fando Maria S., Kostareva Olga S., Tutukina Maria N., Ozoline Olga N., Gelfand Mikhail S., Nikulin Alexey D., Tishchenko Svetlana V.
- Журнал: International Journal of Molecular Sciences
- Том: 23
- Номер: 15
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 8379
- DOI: 10.3390/ijms23158379
- Аннотация: ExuR and UxuR are paralogous proteins belonging to the GntR family of transcriptional regulators. Both are known to control hexuronic acid metabolism in a variety of Gammaproteobacteria but the relative impact of each of them is still unclear. Here, we apply 2D difference electrophoresis followed by mass-spectrometry to characterise the changes in the Escherichia coli proteome in response to a uxuR or exuR deletion. Our data clearly show that the effects are different: deletion of uxuR resulted in strongly enhanced expression of D-mannonate dehydratase UxuA and flagellar protein FliC, and in a reduced amount of outer membrane porin OmpF, while the absence of ExuR did not significantly alter the spectrum of detected proteins. Consequently, the physiological roles of proteins predicted as homologs seem to be far from identical. Effects of uxuR deletion were largely dependent on the cultivation conditions: during growth with glucose, UxuA and FliC were dramatically altered, while during growth with glucuronate, activation of both was not so prominent. During the growth with glucose, maximal activation was detected for FliC. This was further confirmed by expression analysis and physiological tests, thus suggesting the involvement of UxuR in the regulation of bacterial motility and biofilm formation.
- Добавил в систему: Озолинь Ольга Николаевна