|
ИСТИНА |
Войти в систему Регистрация |
Интеллектуальная Система Тематического Исследования НАукометрических данных |
||
Interaction of Bacteroides fragilis Toxin with Outer Membrane Vesicles Reveals New Mechanism of Its Secretion and Deliveryстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Zakharzhevskaya Natalya B., Tsvetkov Vladimir B., Vanyushkina Anna A., Varizhuk Anna M., Rakitina Dania V., Podgorsky Victor V., Vishnyakov Innokentii E., Kharlampieva Dania D., Manuvera Valentin A., Lisitsyn Fedor V., Gushina Elena A., Lazarev Vassili N., Govorun Vadim M.
- Журнал: Frontiers in Cellular and Infection Microbiology
- Том: 7
- Номер: 2
- Год издания: 2017
- Издательство: Frontiers Media S.A.
- Местоположение издательства: Lausanne, Switzerland
- DOI: 10.3389/fcimb.2017.00002
- Аннотация: The only recognized virulence factor of enterotoxigenic Bacteroides fragilis (ETBF) that accompanies bloodstream infections is the zinc-dependent non-lethal metalloprotease B. fragilis toxin (BFT). The isolated toxin stimulates intestinal secretion, resulting in epithelial damage and necrosis. Numerous publications have focused on the interrelation of BFT with intestinal inflammation and colorectal neoplasia, but nothing is known about the mechanism of its secretion and delivery to host cells. However, recent studies of gram-negative bacteria have shown that outer membrane vesicles (OMVs) could be an essential mechanism for the spread of a large number of virulence factors. Here, we show for the first time that BFT is not a freely secreted protease but is associated with OMVs. Our findings indicate that only outer surface-exposed BFT causes epithelial cell contact disruption. According to our in silico models confirmed by Trp quenching assay and NMR, BFT has special interactions with outer membrane components such as phospholipids and is secreted during vesicle formation. Moreover, the strong cooperation of BFT with polysaccharides is similar to the behavior of lectins. Understanding the molecular mechanisms of BFT secretion provides new perspectives for investigating intestinal inflammation pathogenesis and its prevention.
- Добавил в систему: Вишняков Иннокентий Евгеньевич