Manipulating the Performance of Novel Zwitterionic Stationary Phases for HILIC

Chernobrovkina, A.; Kryzhanovskaya, D.; Kovalenko, I.; Shpigun, O.

Авторы: Chernobrovkina Alla, Kryzhanovskaya Darya, Kovalenko Ilya, Shpigun Oleg
Сборник: 33rd International Symposium on Chromatography – ISC 2022
Тезисы
Год издания: 2022
Место издания: Hungarian Society for Separation Sciences, HSSS
Первая страница: c.238
Номер статьи: P-130
Аннотация: Hydrophilic interaction liquid chromatography (HILIC) remains a widely used methodallowing to solve various problems of determination of polar analytes in complex realsamples. Zwitterionic stationary phases are known to provide high separation selectivityfor many classes of substances but still are represented by a limited set of commerciallyavailable columns. However, a variety of spacers and zwitterionic compounds could allowone to manipulate the selectivity of such stationary phases. Revealing the influence ofeach structure fragment on the chromatographic performance of the adsorbents couldhelp to facilitate the creation of new stationary phases with desired properties.In the present work factors affecting selectivity of zwitterionic adsorbents wereinvestigated including the structure of the spacer and the zwitter-ion, the properties ofthe functional groups and the way of their attachment to the substrate, the functionallayer branching. 3-Glycidoxypropyltriethoxysilane and 1,4-butanediol diglycidyl etherwere used as linkers for silica modification. Glycine, N-methylglycine, taurine, Nmethyltaurine, aspartic acid, and iminodiacetic acid were used for creating zwitter-ionicfunctionality in the adsorbents.It was established that using 1,4-butanediol diglycidyl ether as a spacer led to an increasein hydrophilicity, selectivity and efficiency of the phases toward all classes of the test polarsubstances. Introducing glycine zwitter-ion allowed to keep selectivity while increasingmaterial’s hydrophilicity an efficiency toward neutral analytes. Increasing substratecoverage degree resulted in decreasing retention factors and selectivity for acidic analyteswhile keeping anion-exchange selectivity. The obtained results revealed the possibility ofregulating the interaction of zwitterionic stationary phase with target analytes includingion-exchange selectivity.
Добавил в систему: Чернобровкина Алла Валерьевна

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