Yeast red pigment modifies Amyloid beta growth in Alzheimer disease models in both Saccharomyces cerevisiae and Drosophila melanogaster

Nevzglyadova, O.V.; Mikhailova, E.V.; Amen, T.R.; Zenin, V.V.; Artemov, A.V.; Kostyleva, E.I.; Mezhenskaya, D.A.; Rodin, D.I.; Saifitdinova, A.F.; Khodorkovskii, M.A.; Sarantseva, S.V.; Soidla, T.R.

Авторы: Nevzglyadova O.V., Mikhailova E.V., Amen T.R., Zenin V.V., Artemov A.V., Kostyleva E.I., Mezhenskaya D.A., Rodin D.I., Saifitdinova A.F., Khodorkovskii M.A., Sarantseva S.V., Soidla T.R.
Журнал: Amyloid: The Journal of Protein Folding Disorders
Том: 22
Год издания: 2015
Первая страница: 100
Последняя страница: 111
DOI: 10.3109/13506129.2015.1010038
Аннотация: The effect of yeast red pigment on amyloid-β (Aβ) aggregation and fibril growth was studied in yeasts, fruit flies and in vitro. Yeast strains accumulating red pigment (red strains) contained less amyloid and had better survival rates compared to isogenic strains without red pigment accumulation (white strains). Confocal and fluorescent microscopy was used to visualise fluorescent Aβ-GFP aggregates. Yeast cells containing less red pigment had more Aβ-GFP aggregates despite the lower level of overall GFP fluorescence. Western blot analysis with anti-GFP, anti-Aβ and A11 antibodies also revealed that red cells contained a considerably lower amount of Aβ GFP aggregates as compared to white cells. Similar results were obtained with exogenous red pigment that was able to penetrate yeast cells. In vitro experiments with thioflavine and TEM showed that red pigment effectively decreased Aβ fibril growth. Transgenic flies expressing Aβ were cultivated on medium containing red and white isogenic yeast strains. Flies cultivated on red strains had a significant decrease in Aβ accumulation levels and brain neurodegeneration. They also demonstrated better memory and learning indexes and higher locomotor ability.
Добавил в систему: Костылева Елена Ивановна

	ИСТИНА	Войти в систему Регистрация
	Интеллектуальная Система Тематического Исследования НАукометрических данных
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

Интеллектуальная Система Тематического Исследования НАукометрических данных

Yeast red pigment modifies Amyloid beta growth in Alzheimer disease models in both Saccharomyces cerevisiae and Drosophila melanogasterстатья