Pump and Channel K+ (Rb+) Fluxes in Apoptosis of Human Lymphoid Cell Line U937

Vereninov, A.; Rubashkin, A.; Goryachaya, T.; Moshkov, A.; Rozanov, Y.; Shirokova, A.; Strelkova, E.; Lang, F.; Yurinskaya, V.

Авторы: Vereninov Alexey, Rubashkin Andrey, Goryachaya Tatyana, Moshkov Alexey, Rozanov Yuriy, Shirokova Anna, Strelkova Ekaterina, Lang Florian, Yurinskaya Valentina
Журнал: Cellular Physiology and Biochemistry
Том: 22
Номер: 1-4
Год издания: 2008
Издательство: S. Karger AG
Местоположение издательства: Switzerland
Первая страница: 187
Последняя страница: 194
DOI: 10.1159/000149796
Аннотация: Ouabain-sensitive (OS) and -resistant (OR) Rb(+) influx was examined in three sublines of U937 cells to compare alterations of K(+) channel permeability and the Na(+),K(+)-ATPase pump leading to the shift in ion and water balance during apoptosis induced by 0.2 and 1microM staurosporine (STS) for 4-5 h. Cell K(+), Rb(+), Na(+) and Cl(-) content was determined by flame photometry and (36)Cl distribution. Changes in cell water content were monitored by measurement of buoyant cell density and distribution of [(3)H]-glycerol or 3-O-methyl-D-[(3)H]glucose. Apoptosis was detected by DNA flow cytometry and light microscopy of the native cells stained with acridine orange. Treatment with 0.2 microM STS for 5 hours led to mild apoptosis with 10-13 % cell dehydration and either moderate increase of channel mediated Rb(+) influx without significant changes in the pump activity or moderate decrease of pump Rb(+) influx without significant change of channel influx, depending on the cell line used. Treatment with 1 microM STS was followed by 18-23 % cell dehydration, a decrease of the pump activity and a small or insignificant increase in the OR Rb(+) influx in all studied sublines. It is concluded that moderate apoptotic cell shrinkage may be associated with both an increase in K(+) channel permeability and inhibition of the pump whereas more remarkable shrinkage occurs presumably due to inhibition of the pump.
Добавил в систему: Мошков Алексей Владимирович

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Pump and Channel K+ (Rb+) Fluxes in Apoptosis of Human Lymphoid Cell Line U937статья

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