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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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A Three-dimensional Model of the Human Facilitative Glucose Transporter Glut1статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 8 сентября 2021 г.
- Авторы: Zuniga Felipe A., Guangpu Shi, Haller Jorge F., Andrey Rubashkin, Flynn Diana R., Pavel Iserovich, Jorge Fischbarg
- Журнал: Journal of Biological Chemistry
- Том: 276
- Номер: 48
- Год издания: 2001
- Издательство: American Society for Biochemistry and Molecular Biology Inc.
- Местоположение издательства: United States
- Первая страница: 44970
- Последняя страница: 44975
- DOI: 10.1074/jbc.M107350200
- Аннотация: The human facilitative transporter Glut1 is the major glucose transporter present in all human cells, has a central role in metabolism, and is an archetype of the superfamily of major protein facilitators. Here we describe a three-dimensional structure of Glut1 based on helical packing schemes proposed for lactose permease and Glut1 and predictions of secondary structure, and refined using energy minimization, molecular dynamics simulations, and quality and environmental scores. The Ramachandran scores and the stereochemical quality of the structure obtained were as good as those for the known structures of the KcsA K+ channel and aquaporin 1. We found two channels in Glut1. One of them traverses the structure completely, and is lined by many residues known to be solvent-accessible. Since it is delimited by the QLS motif and by several well conserved residues, it may serve as the substrate transport pathway. To validate our structure, we determined the distance between these channels and all the residues for which mutations are known. From the locations of sugar transporter signatures, motifs, and residues important to the transport function, we find that this Glut1 structure is consistent with mutagenesis and biochemical studies. It also accounts for functional deficits in seven pathogenic mutants.
- Добавил в систему: Рубашкин Андрей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | English text | 3_Dim_Model_Glut1-J_Biol_Chem-2001.pdf | 506,8 КБ | 10 августа 2017 [RubashkinAA] |