Аннотация:The reconstruction of the evolutionary history of sex determination in squamate reptiles(lizards and snakes) is complicated by missing data in many lineages, erroneousreports, and often questionable inferences on state homology. Therefore, despite thelarge effort, the reconstruction of the ancestral sex determination in squamate reptilesis still controversial. With the hope to shed light on this problem, we aspired toidentify the sex chromosome gene content in Dibamus deharvengi, the representativeof the family Dibamidae, the putative sister clade to all other squamates. Our analysesrevealed XX/XY sex-determinationsystem in D. deharvengi: the X chromosomecontains genes with homologues scattered across chicken chromosomes 8, 12, 13,18, 30, and 33, and the Y chromosome seems to largely degenerate. To the best ofour knowledge, this combination has never been reported to form sex chromosomesin any amniote lineage. It suggests that the sex chromosomes can represent an apomorphyof a clade including D. deharvengi. Our findings cover an important gap in the knowledge of sex determination in reptiles and further support multiple independent origins of sex chromosomes in this group.