Gamma globulins-induced interaction between two red blood cells: forces measurement with optical tweezersстатья

Информация о цитировании статьи получена из Scopus, Web of Science
Дата последнего поиска статьи во внешних источниках: 14 августа 2017 г.

Работа с статьей


[1] Gamma globulins-induced interaction between two red blood cells: forces measurement with optical tweezers / K. Lee, A. Muravyov, A. Semenov et al. // Proceedings of SPIE. — Vol. 10336 of Proceedings of Saratov Fall Meeting 2016: Optical Technologies in Biophysics & Medicine XVII. — Bellingham, WA, United States: Bellingham, WA, United States, 2017. — P. 10336056. Aggregation of red blood cells (RBCs) is an intrinsic property of blood, which has direct effect on the blood viscosity and therefore affects overall the blood circulation throughout the body. It is attracting interest for the research in both fundamental science and clinical application. Despite of the intensive research, the aggregation mechanism is remaining not fully clear. Recent advances in methods allowed measuring the interaction between single RBCs in a well-defined configuration leading to the better understanding of the mechanism of the process. However the most of the studies were made on the static cells. Thus, the measurements in flow mimicking conditions are missing. In this work, we aim to study the interaction of two RBCs in the flow conditions. We demonstrate the characterization of the cells interaction strength (or flow tolerance) by measuring the flow velocity to be applied to separate two aggregated cells trapped by double channel optical tweezers in a desired configuration. The age-separated cells were used for this study. The obtained values for the minimum flow velocities needed to separate the two cells were found to be 78.9 ± 6.1 μm/s and 110 ± 13 μm/s for old and young cells respectively. The data obtained is in agreement with the observations reported by other authors. The significance of our results is in ability for obtaining a comprehensible and absolute physical value characterizing the cells interaction in flow conditions (not like the Aggregation Index measured in whole blood suspensions by other techniques, which is some abstract parameter). Keywords: Red blood cell, aggregation, optical tweezers, flow, interaction strength, age, single cell. [ DOI ]

Публикация в формате сохранить в файл сохранить в файл сохранить в файл сохранить в файл сохранить в файл сохранить в файл скрыть