Newsletter of the Russian Society of Nematologists Abstracts of the Eleventh International Symposium of the Russian Society of Nematologists: Panagrolaimid nematodes suppressing the laboratory cultures of drosophilid flies in insectariaстатья Научная хроника Тезисы

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[1] IVANOVA E. S., PERFILIEVA K. S., SPIRIDONOV S. E. Newsletter of the russian society of nematologists abstracts of the eleventh international symposium of the russian society of nematologists: Panagrolaimid nematodes suppressing the laboratory cultures of drosophilid flies in insectaria // Russian Journal of Nematology. — 2015. — Vol. 23, no. 2. — P. 158–158. The suppression of the laboratory lines of Drosophila kept at the Department of Biological Evolution of the Moscow State University in the period from August 2013 up to June 2014 was induced by the mass development of unknown, small-sized nematodes in the nutrient medium in the culture flasks. The nematodes were washed out from the vials and used for the morphological and molecular-taxonomic study. The study of nematodes carried out by light and scanning microscopy revealed the characteristic features of panagrolaimid nematodes. Nucleotide sequences of the partial SSU rDNA (approx. 1000 bp ) and D2-D3 segment of LSU rDNA (approx. 1100 bp) were obtained with primer pairs Nem18SF (CGC GAA TRG CTC ATT ACA ACA GC)-Nem18SR (GGG CGG TAT CTG ATC GCC) and LSU531 (CTT CGC AAT GAT AGG AAG AGC C)-LSU537 (GAT CCG TAA CTT CGG GAA AAG GAT), respectively. Sequences obtained were used for BLAST-search in NCBI GenBank and the affinity with a set of rhabditid nematodes was revealed. All the similar sequences were used to construct alignments (862 bp long for SSU and 951 bp long for LSU). Three methods of analysis demonstrate the similarity of the sequence of nematode from Drosophila cultures with panagrolaimids: Panagrellus redividus and P. dubius in the LSU rDNA sequence and P. redivivus, Panagrolaimus paetzoldi and Baujardia mirabilis in the SSU rDNA. Both in SSU and LSU alignments, the difference between the species under study and Panagrellus redivivus was the lowest (46 bp and 39 bp correspondingly). In the LSU rDNA tree, the nematode from drosophilid cultures also clusters with a clade consisting of several panagrolaimid genera. Despite the revealed similarity, the nematodes studied cannot be assigned to the genera Panagrellus, Panagrolaimus or Baujardia forming a sister taxon to the clade of these three genera. The wider molecular-taxonomic study of panagrolaimids is needed for identification of this nematode (Support: RFBR 14-04- 01513-a). – 1 Centre of Parasitology, A.N. Severtsov Institute of Ecology and Evolution, RAS, Moscow, 119071, Russia. 2 Biological Faculty, Lomonosov Moscow State University, Moscow, 119991, Russia; E-mail: ksenperf@mail.ru. http://www.sevin.ru/Helminths/pdf/10.pdf.

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