Аннотация:The present study aimed to evaluate in vitro enzymatic activity of a novel biotechnologicalactive complex based on natural origin compounds—thermophilic lipase and β-cyclodextrin—forhydrolysis of acylglycerides in plant oils and fats. β-cyclodextrin (β-CD) as an additive has attractedattention for enhance stability and efficiency of enzymes. In present study, the effects of β-CD onenzymatic hydrolysis of acylglycerides by thermophilic lipase were investigated by modern methods. The UV-spectroscopy, electron microscopy with TEM and kinetics of enzymatic hydrolysiswere compared by the addition of β-CD, respectively. The results showed that lipase could producethe highest yield of oleic acid in presence of β-CD after 1 and 3 h. The UV spectroscopy demonstrated that the absorbance and fluorescence of lipase decreased with increasing concentration of βCD due to surface interaction and change of enzyme configuration. Moreover, electron microscopywith TEM showed that lipase formed a special active conglomerate with β-CD for improving hydrolysis and stability. Dermatology evaluation indicated that complex added to household productsdidn’t affect sensitive skin of hands. Overall results indicate that β-CD could increase enzymaticactivity of the lipase against acylglycerides and can be considered as promising composition in ecological household products for regular hand application.
