Cryostructuring of Polymeric Systems: 63. † Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian Cells

Lozinsky, V.I.; Kulakova, V.K.; Grigoriev, A.M.; Podorozhko, E.A.; Kirsanova, L.A.; Kirillova, A.D.; Novikov, I.A.; Basok, Y.B.; Sevastianov, V.I.

Авторы: Lozinsky Vladimir I., Kulakova Valentina K., Grigoriev Alexei M., Podorozhko Elena A., Kirsanova Ludmila A., Kirillova Aleksandra D., Novikov Ivan A., Basok Yulia B., Sevastianov Viktor I.
Журнал: GELS
Том: 8
Номер: 11
Год издания: 2022
Издательство: MDPI Publishing
Местоположение издательства: Basel, Switzerland, Switzerland
Номер статьи: 695
DOI: 10.3390/gels8110695
Аннотация: Various gelatin-containing gel materials are used as scaffolds for animal and human cellculturing within the fields of cell technologies and tissue engineering. Cryostructuring is a promisingtechnique for the preparation of efficient macroporous scaffolds in biomedical applications. In thecurrent study, two new gelatin-based cryostructurates were synthesized, their physicochemicalproperties and microstructure were evaluated, and their ability to serve as biocompatible scaffoldsfor mammalian cells culturing was tested. The preparation procedure included the dissolution ofType A gelatin in water, the addition of urea to inhibit self-gelation, the freezing of such a solution,ice sublimation in vacuo, and urea extraction with ethanol from the freeze-dried matter followedby its cross-linking in an ethanol medium with either carbodiimide or glyoxal. It was shown thatin the former case, a denser cross-linked polymer phase was formed, while in the latter case, themacropores in the resultant biopolymer material were wider. The subsequent biotesting of thesescaffolds demonstrated their biocompatibility for human mesenchymal stromal cells and HepG2 cellsduring subcutaneous implantation in rats. Albumin secretion and urea synthesis by HepG2 cellsconfirmed the possibility of using gelatin cryostructurates for liver tissue engineering.
Добавил в систему: Лозинский Владимир Иосифович

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Cryostructuring of Polymeric Systems: 63. † Synthesis of Two Chemically Tanned Gelatin-Based Cryostructurates and Evaluation of Their Potential as Scaffolds for Culturing of Mammalian Cellsстатья

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