A microchip electrophoresis-assisted triple-cycle cascade chemiluminescence signal amplification strategy for the ultrasensitive detection of microRNA-141 in cells

Авторы: Xing Yang, Jingjin Zhao, Li Hou, Ivan Yu Sakharov, Jianniao Tian, Shulin Zhao
Журнал: Talanta
Том: 253
Год издания: 2023
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Номер статьи: 124011
DOI: 10.1016/j.talanta.2022.124011
Аннотация: Based on the microchip electrophoresis chemiluminescence detection (MCE-CL) platform, an ultrasensitivemethod was developed for the detection of microRNA-141 (miRNA-141) by using T7 exonuclease (T7 Exo)-assisted triple-cycle cascade chemiluminescence signal amplification strategy. In this method, the horseradishperoxidase (HRP)-labeled single strand DNA (HRP-DNA) was used as a signal probe for the cascade signalamplification identification of miRNA-141 in cancer cells. Under the optimized experimental conditions, thechemiluminescence intensity and logarithm of the miRNA-141 concentration showed a good linear relationship.The detection limit for miRNA-141 assay reached 9.1 × 10􀀀 17 M (S/N = 3). This method displayed excellentspecificity for differentiating miRNAs with base mismatch, and was applied to determine miRNA-141 in thelysates of cancer cells. This method shows important applications when using limited amounts of biologicalsamples for clinical diagnostics and biomedical research of miRNAs.
Добавил в систему: Сахаров Иван Юрьевич

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