Double Immunochromatographic Test System for Sensitive Detection of Phycotoxins Domoic Acid and Okadaic Acid in Seawater and Seafood

Авторы: Hendrickson Olga D., Zvereva Elena A., Solopova Olga N., Zherdev Anatoly V., Sveshnikov Peter G., Eremin Sergei A., Dzantiev Boris B.
Журнал: MICROMACHINES
Том: 13
Номер: 9
Год издания: 2022
Первая страница: 1506
Номер статьи: 1506
DOI: 10.3390/mi13091506
Аннотация: In this investigation, a double immunochromatographic analysis (ICA) of two relevant phycotoxins, domoic acid (DA) and okadaic acid (OA), was developed for the first time. The ICA was performed in the indirect competitive format using gold nanoparticles conjugated with antispecies antibodies. Under optimal conditions, the instrumental detection limits/cutoffs for simultaneous detection of DA and OA were 1.2/100 and 0.1/2.5 ng/mL, respectively. The time of the assay was 18 min. The ICA was applied to test seawater and a large panel of seafood, including mussels, tiger shrimps, octopuses, whelks, crabs, and scallops. The proposed simple sample preparation method for seafood takes only 20 min. For seawater, a dilution by buffer was implemented. The assay recoveries varied from 80.8% to 124.5%. The competitive potential of the proposed technique as a tool to control natural water and seafood samples is determined by its simplicity, rapidity, and sensitivity.
Добавил в систему: Еремин Сергей Александрович

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