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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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In vitro and in vivo antimicrobial activity of an active plant-based quadrocomplex for skin hygieneстатья Исследовательская статья
- Авторы: Filatov VA, Kulyak OY, Kalenikova EI
- Журнал: JOURNAL OF PHARMACY & PHARMACOGNOSY RESEARCH
- Том: 10
- Номер: 5
- Год издания: 2022
- Первая страница: 905
- Последняя страница: 921
- DOI: 10.56499/jppres22.1452_10.5.905
- Аннотация: Context: The current epidemiological situation causes a new surge of interest to perspective antimicrobial formulations for proper skin hygiene.Aims: To evaluate in vitro and in vivo the antimicrobial activity of a novel active quadrocomplex (QC) for skin hygiene based on Melaleuca alternifolia essentialoil, eucalyptol, (-)-α-bisabolol and silver citrate. In addition, to analyze the phytochemical constituents by gas chromatography-mass spectrometry (GC-MS)and to assess the skin irritant potential after regular washing.Methods: The phytochemical analysis was performed using GC-MS. The Minimum Inhibitory Concentrations (MICs) were assessed using a colony-countingmethod with resazurin. The type of pharmacological interaction was investigated using a modern checkerboard assay.Results: The chemical composition exhibited 18 resolved phytochemicals with the highest concentrations for (-)-α-bisabolol (32.2%) and terpinen-4-ol (31.6%)through the GC-MS analysis. QC agents showed antimicrobial activity against Gram-positive and Gram-negative strains, with MIC values ranging from 1.25 to40.00 mg/mL. The checkerboard assay demonstrated reduced MIC values for the combinations of QC agents against all reference strains. QC showedsignificant inhibition of Staphylococcus aureus growth with an average efficiency of 99.91% and Candida albicans 99.94 %. In vivo, the investigation of QC showed higher immediate and prolonged efficiency compared to base formulation (p<0.05). Dermatology evaluation indicated that QC added to the soap base did not affect the skin of hands during regular hand-washing and did not cause any negative effects on the skin.Conclusions: QC exhibits a balanced performance between antimicrobial activity and biological safety and can be considered a promising bioactivecomposition for regular hand hygiene application. Therefore, additional investigations are needed to study the mechanism of antimicrobial activity.Keywords: antimicrobial agents; drug synergism; Gram-negative pathogens; Gram-positive pathogens; phytochemicals; skin microbiota.
- Добавил в систему: Каленикова Елена Игоревна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | jppres22.1452_10.5.905.pdf | jppres22.1452_10.5.905.pdf | 580,8 КБ | 12 сентября 2022 [Elena_Kalenikova] | |
| 2. | Полный текст | jppres22.1452_10.5.905_zi4oIIr.pdf | 580,8 КБ | 12 сентября 2022 [Elena_Kalenikova] |