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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Implantable graded-index fibers for neural-dynamics-resolving brain imaging in awake mice on an air-lifted platformстатья
- Авторы: Pochechuev Matvey S., Fedotov Ilya V., Martynov Grigoriy N., Solotenkov Maxim A., Ivashkina Olga I., Rogozhnikova Olga S., Fedotov Andrei B., Anokhin Konstantin V., Zheltikov Aleksei M.
- Журнал: Journal of Biophotonics
- Год издания: 2022
- Издательство: Wiley - VCH Verlag GmbH & CO. KGaA
- Местоположение издательства: Germany
- Номер статьи: e202200025
- DOI: 10.1002/jbio.202200025
- Аннотация: We demonstrate a versatile framework for cellular brain imaging in awake mice based on suitably tailored segments of graded-index (GRIN) fiber. Closed-form solutions to ray-path equations for graded-index waveguides are shown to offer important insights into image-transmission properties of GRIN fibers, suggesting useful recipes for optimized GRIN-fiber-based deep-brain imaging. We show that the lengths of GRIN imaging components intended for deep-brain studies in freely moving rodents need to be chosen as a tradeoff among the spatial resolution, the targeted imaging depth and the degree of fiber-probe invasiveness. In the experimental setting that we present in this paper, the head of an awake mouse with a GRIN-fiber implant is fixed under a microscope objective, but the mouse is free to move around an in-house-built flat-floored air-lifted platform, exploring a predesigned environment, configured as an arena for one of standard cognitive tests. We show that cellular-resolution deep-brain imaging can be integrated in this setting with robust cell-specific optical neural recording to enable in vivo studies with minimal physical restraints on animal models. The enhancement of the information capacity of the fluorescence signal, achieved via a suitable filtering of the GRIN-fiber readout, is shown to open routes toward practical imaging modalities whereby the deep-brain neuronal dynamics and axonal connections underpinning the integrative functions of essential brain structures can be studied in awake rodent models.
- Добавил в систему: Ивашкина Ольга Игоревна