Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albumin

Mikhalchik, E.V.; Ivanov, V.A.; Borodina, I.V.; Pobeguts, O.V.; Smirnov, I.P.; Gorudko, I.V.; Grigorieva, D.V.; Boychenko, O.P.; Moskalets, A.P.; Klinov, D.V.; Panasenko, O.M.; Filatova, L.Y.; Kirzhanova, E.A.; Balabushevich, N.G.

Авторы: Mikhalchik E.V., Ivanov V.A., Borodina I.V., Pobeguts O.V., Smirnov I.P., Gorudko I.V., Grigorieva D.V., Boychenko O.P., Moskalets A.P., Klinov D.V., Panasenko O.M., Filatova L.Y., Kirzhanova E.A., Balabushevich N.G.
Журнал: International Journal of Molecular Sciences
Том: 23
Номер: 14
Год издания: 2022
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 1
Последняя страница: 14
Номер статьи: 7840
DOI: 10.3390/ijms23147840
Аннотация: Hyperglycemia-induced protein glycation and formation of advanced glycation end-products (AGEs) plays an important role in the pathogenesis of diabetic complications and pathological biomineralization. Receptors for AGEs (RAGEs) mediate the generation of reactive oxygen species (ROS) via activation of NADPH-oxidase. It is conceivable that binding of glycated proteins with biomineral particles composed mainly of calcium carbonate and/or phosphate enhances their neutrophil-activating capacity and hence their proinflammatory properties. Our research managed to confirm this hypothesis. Human serum albumin (HSA) was glycated with methylglyoxal (MG), and HSA-MG was adsorbed onto mineral microparticles composed of calcium carbonate nanocrystals (vaterite polymorph, CC) or hydroxyapatite nanowires (CP). As scopoletin fluorescence has shown, H2O2 generation by neutrophils stimulated with HSA-MG was inhibited with diphenyleneiodonium chloride, wortmannin, genistein and EDTA, indicating a key role for NADPH-oxidase, protein tyrosine kinase, phosphatidylinositol 3-kinase and divalent ions (presumably Ca2+) in HSA-MG-induced neutrophil respiratory burst. Superoxide anion generation assessed by lucigenin-enhanced chemiluminescence (Luc-CL) was significantly enhanced by free HSA-MG and by both CC-HSA-MG and CP-HSA-MG microparticles. Comparing the concentrations of CC-bound and free HSA-MG, one could see that adsorption enhanced the neutrophil-activating capacity of HSA-MG.
Добавил в систему: Балабушевич Надежда Георгиевна

	ИСТИНА	Войти в систему Регистрация
	Интеллектуальная Система Тематического Исследования НАукометрических данных
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

Интеллектуальная Система Тематического Исследования НАукометрических данных

Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albuminстатья

Прикрепленные файлы