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Optimization of Enzyme Assisted Alkaline Extraction of Sunflower (Helianthus Annuus L.) Protein for Alternative Isolate Productionстатья
Дата последнего поиска статьи во внешних источниках: 20 июля 2022 г.
- Авторы: Baurin D.V., Baurina A.V., Shakir I.V., Panfilov V.I.
- Журнал: Chemical Engineering Transactions
- Том: 93
- Год издания: 2022
- Первая страница: 175
- Последняя страница: 180
- DOI: 10.3303/CET2293030
- Аннотация: This research focused on the effect of extraction time (60–120 min), enzyme/substrate ratio (0.05–0.4 %) temperature (37.5°C) on enzyme assisted alkaline protein extraction efficiency from non-dehulled sunflower meal, which is a by-product of oil production. 4 commercial enzymes were studied and the highest protein yield (92 %) was obtained by ultrafiltration of the enzymatic hydrolysates through a membrane (selectivity 81.4%) followed by the isoelectric precipitation (pH 4.5) and vacuum drying. The protein yield was found 92.85% at the optimized condition of enzyme assisted alkaline extraction: temperature 37.5 °C, 60 min, and enzyme/substrate ratio of 0.2%. Compared to alkaline extraction with 0.25% NaOH and no enzyme addition, the protein yield has increased by 31% due to the mild extraction conditions. Determined physicochemical properties of the obtained protein isolate: high protein content (92.85% d.b) as well as high solubility (95.0% at pH 7.5) reflect the unique characteristics of the product. However, the process requires further optimization because of the green colour of the isolate, which limits its application in the food industry.
- Добавил в систему: Баурина Александра Владимировна
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