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Dynamics of human telomerase RNA structure revealed by antisense oligonucleotide techniqueстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 мая 2014 г.
- Авторы: Vasilkova DP, Azhibek DM, Zatsepin TS, Naraikina YV, Prassolov VS, Prokofjeva MM, Zvereva MI, Rubtsova MP
- Журнал: Biochimie
- Том: 95
- Номер: 12
- Год издания: 2013
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 2423
- Последняя страница: 2428
- DOI: 10.1016/j.biochi.2013.09.001
- Аннотация: Telomeres are the nucleoprotein complexes that cap the linear chromosome ends. Telomerase is a ribonucleoprotein that maintains telomere length in stem, embryonic and cancer cells. Somatic cells don’t contain active telomerase and telomere function as mitotic clock and telomere length determines the number of cell divisions. Telomerase RNA (TER) contains the template for telomere synthesis and serves as a structural scaffold for holoenzyme assembly. We compared different oligonucleotide based methods for telomerase RNA inhibition, such as antisense oligonucleotides, knockdown by transient siRNA transfection and silencing by miRNA derived from short expressed RNA hairpin in HEK293 cells. All of these methods were applied to different TER regions. Our results revealed that CR2/CR3 domain of TER is accessible in vitro and in vivo and could serve as an optimal site for oligonucleotide-based telomerase silencing.
- Добавил в систему: Рубцова Мария Петровна