Аннотация:Telomerase is the essential enzyme, that maintains telomere length in eukaryotes. Telomeres are essential for genome stability, protecting natural chromosome ends against fusion, recombination and degradation. The telomerase ribonucleoprotein is composed of two core components, telomerase RNA (hTR in humans) and reverse transcriptase protein subunit (hTERT in humans). The study of the processing of human telomerase RNA is complicated by its low content in cells.
We created stable cell lines with overexpression the major components of telomerase. The expression level of hTR and hTERT mRNA was analysed by qRT-PCR. The level of hTR increased in presence of hTERT. It allows to propose the reverse transcriptase is necessary for hTR stabilization.
Also we created stable cell line with overexpression mutant telomerase RNA, that had a deletion of two nucleotides in 3’-end sequence. Our data show that mutant form of telomerase RNA is processed better than wild-type hTR.