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Nanostructured polyelectrolyte films for engineering highly sensitive tyrosinase biosensors: specifics of enzyme-polyelectrolyte structuresстатья
Дата последнего поиска статьи во внешних источниках: 17 февраля 2021 г.
- Авторы: Dubacheva G.V., Porus M.V., Sigolaeva L.V., Pergushov D.V., Tur D.R., Papkov V.S., Zezin A.B., Yaroslavov A.A., Eremenko A.V., Kurochkin I.N., Varfolomeev S.D.
- Журнал: Nanotechnologies in Russia
- Том: 3
- Номер: 3-4
- Год издания: 2008
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 221
- Последняя страница: 227
- DOI: 10.1134/S1995078008030099
- Аннотация: The correlation was studied between the activity of tyrosinase electrodes engineered by layer-by-layer deposition technology and the number of PDDA/tyrosinase layers (where PDDA stands for poly(diallyldimethylammonium chloride)). The last deposited layer is the active one regardless of the number of layers. Atomic force microscopy (AFM) characterization of the topology of (PDDA/tyrosinase)1 and (PDDA/tyrosinase)2 films showed that these films have identical structures. The operational stability of tyrosinase biosensors coated with (PDDA/tyrosinase)1 films was examined. Various methods for stabilizing tyrosinase biosensors were tested. Crosslinking with glutaraldehyde (GA) improved 2.5-fold the operational stability of (PDDA/tyrosinase)1 films.
- Добавил в систему: Пергушов Дмитрий Владимирович