Spatial localization of chromosome-nuclear envelope interaction sitesстатья

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Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.

Работа с статьей

[1] Spatial localization of chromosome-nuclear envelope interaction sites / E. A. Vladimirskaya, I. I. Kireyev, A. N. Prusov, D. Fais // Membrane and Cell Biology. — 1998. — Vol. 12, no. 6. — P. 857–869. In the interphase nucleus chromosomes are tightly associated with the nuclear envelope (NE) through special granular chromatin particles termed anchorosomes. It remains unclear whether anchorosomes represent constant nuclear structures, persisting throughout the cell cycle, or they appear only in the interphase during the formation of contacts between the chromosomes and NE. In other words, whether specific NE interaction sites do exist in chromosomes or any region can form anchorosome. In this work, we used micrononucleated PK cells, in which almost every micronucleus (MN) is formed by a single chromosome. The spatial distribution and quantitative characteristics of the anchorosomal layer in MN was studied using stereological analysis and three-dimensional computer reconstruction. It was shown that in cells with about 30 MN, the total surface area of NE reaches about 355 Ојm2, whereas in normal mononuclear cells it is 110 Ојm2. Hence, the NE surface increases 3-fold during MN formation. In contrast to normal cells, only 80% of the NE surface in MN is covered with anchorosomes, i.e. the total surface area of the anchorosomal layer increases by a factor of 2.5. The 3D reconstruction has demonstrated highly random distribution of anchorosome-free zones, the distribution patterns varying in individual MN. These findings are thought to be evidence for the existence of a limited number of specific chromosomal sites potentially capable of forming contacts with NE.

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