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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Study of lysozyme by a spin-label method in the 2-mm rangeстатья
- Авторы: Krinichnyi V.I., Grinberg O.Y., Iudanova E.I., Liubashevskaia E.V., Antsiferova L.I.
- Журнал: Biophysics
- Том: 32
- Номер: 2
- Год издания: 1987
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 215
- Последняя страница: 220
- Аннотация: Two millimeter range ESR-spectroscopy was used to measure the values of magnetic resonance parameters of egg lysozyme samples modified with nitroxyl label 4-(iodine-acetamide)-2,2,6,6-tetramethylpiperidine-1- oxyl by hist-15 residue. It has been shown that in a lyophilic sample the spin label forms a hydrogen bond with the protein group and when the sample is moistened--with water molecules. Temperature changes of the pattern of ESR line are analysed. It is concluded that in the moistened samples within 230-320 K the nitroxyl fragment of the label gets engaged in anisotropic movement with preferable rotation around Z- axis of N-O. fragment with anisotropy coefficient 5 and mean correlation time tau congruent to 5 X 10(-7) divided by 5 X 10(-8) c
- Добавил в систему: Криничный Виктор Иванович