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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Glycopolymers of the Cell Walls in Classification of Representatives of the Cluster Streptomyces albusстатья Тезисы
- Авторы: Tul'skaya E.M., Streshinskaya G.M., Shashkov A.S., Senchenkova S.N., Dmitrenok A.S., Baryshnikova L.M.
- Журнал: Achievements in the Life Sciences
- Том: 10
- Год издания: 2016
- Первая страница: S54
- DOI: 10.1016/j.als.2016.12.053
- Аннотация: The structures of the glycopolymers were established using a combination of chemical and NMR spectroscopicmethods. The cell wall of S. albus subsp. albus VKM Ac-35T was found to be comprised of three glycopolymers, viz. unsubstituted 1,5-poly(ribitol phosphate), 1,3-poly(glycerol phosphate) substituted with β-D-glucopyranose, and the major polymer, a Kdn (3-deoxy-D-glycero-D-galacto-non-2-ulosonic acid)-teichulosonic acid: β-D-Glcp-(1 → 8)-α-Kdnp-(2[(→6)-β-D-Glcp-(1 → 8)-α-Kdnp-(2→]n 6)-β-D-Glcp-(1 → 8)-β-Kdnp-(2-OH, where n ≥ 3. The cell walls of ‘S. albus’ J1074 and ‘S. albus’ R1–100 were found to contain three glycopolymers of identical structures,viz.unsubstituted1,3-and2,3-poly(glycerolphosphates), and the major polymer, a Kdn-teichulosonic acid with an unusual structure that has not been previously described: β-D-Galp-(1 → 9)-α-Kdnp-(2[(→3)-β-D-Galp-(1 → 9)-α-Kdnp-(2→]n 3)-β-D-Galp-(1 → 9)-β-Kdnp-(2-OH, where n ≥ 7–8.Thecellwallof Streptomyces pathocidini (formerly S. albus subsp. pathocidicus VKM Ac-598T)was found to contain two glycopolymers, viz. 1,3-poly(glycerol phosphate) partially O-glycosylated with 2-acetamido-2-deoxy-α-D-glucopyranose and/or O-acylated with L-lysine, and a poly(diglycosyl 1-phosphate) of hitherto unknown structure: -6)-α-D-Glcp-(1 → 6)-α-D-GlcpNAc-(1-P-.This study showed that the related strains ‘S. albus’ J1074 and R1-100 [sensitive and resistant, respectively, to moenomycin A, which is a phosphoglycolipid antibiotic that inhibits the biosynthesis of peptidoglycan (Ostash and Walker, 2010)] contain the same composition and structure of glycopolymers. Thus the sensitivity/resistance to moenomycin A does not depend on the composition and structure of the cell wall glycopolymers.The type strain of S. albus, VKM Ac-35T , was found to have a distinct profile of cell wall glycopolymers. These data support the conclusion that ‘S. albus’ J1074 and R1-100 belong to a distinct species, S. albidoflavus (Labeda et al., 2014). Likewise, the reclassification of S. albus subsp. pathocidicus VKM Ac-598T as a novel species, S. pathocidini VKM Ac-598T (Labeda et al., 2014)is supported by the differences between its cell wall glycopolymer profile and that of S. albus VKM Ac-35T . Taking into account all the data discussed above, the value of determining the structure and composition of cell wall glycopolymers for the taxonomy and species specificity of the members of the genus Streptomyces becomes evident.
- Добавил в систему: Тульская Елена Михайловна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Краткий текст | Gly_pol_S_albus_2017_JuyA6c1.pdf | 93,3 КБ | 17 февраля 2017 [TulskayaEM] | |
| 2. | Краткий текст | Gly_pol_S_albus_2017.pdf | 93,3 КБ | 17 февраля 2017 [TulskayaEM] |