Compaction and transmembrane delivery of pDNA: differences between l-PEI and two types of amphiphilic block copolymersстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 апреля 2017 г.
Авторы:
Raup A.,
Wang H.,
Synatschke C.V.,
Jérôme V.,
Agarwal S.,
Pergushov D.V.,
Müller A.H.E,
Freitag R.
Аннотация:Polycations are popular agents for non-viral delivery of DNA to mammalian cells. Adding hydrophobic, biodegradable, or cell penetrating functions could help to improve their performance, which at present is below that of viral agents. A crucial first step in gene delivery is the complexation of the DNA. The characteristics of these ‘polyplexes’ presumably influence or even determine the subsequent steps of membrane passage, intracellular travelling / DNA release, and nuclear up-take. Herein, polyplexes formed with linear poly(ethylenimine) (l-PEI) are compared to complexes generated with functionalized diblock copolymers. While l-PEI interacts only electrostatically with the DNA, interaction in case of the diblock polymers may be mixed-mode. In certain cases transfection efficiency improved when the polyplexes were formed in hypertonic solution. Moreover, whereas conventional PEI-based polyplexes enter the cells via endocytosis, at least one of the diblock agents seemed to promote entry via transient destabilization of the plasma membrane.