Effects of copper on viability and functional properties of hippocampal neurons in vitroстатья

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Дата последнего поиска статьи во внешних источниках: 12 июля 2017 г.

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[1] Effects of copper on viability and functional properties of hippocampal neurons in vitro / M. R. Kapkaeva, O. V. Popova, R. V. Kondratenko et al. // Experimental and Toxicologic Pathology. — 2017. — Vol. 69. — P. 259–264. Copper (Cu2+) is an essential metal presented in the mammalian brain and released from synaptic vesicles following neuronal depolarization. However, the disturbance of Cu2+ homeostasis results in neurotoxicity. In our study we performed for the first time a combined functional investigation of cultured hippocampal neurons under Cu2+ exposure, its effect on spontaneous spike activity of hippocampal neuronal network cultured on multielectrode array (MEA), and development of long-term potentiation (LTP) in acute hippocampal slices in the presence of Cu2+. Application of 0.2 mM CuCl2 for 24 h reduced viability of cultured neurons to 40+/-6%, whereas 0.01 mM CuCl2 did not influence significantly on the neuronal survival. However, exposure to the action of 0.01 mM Cu2+ resulted in pronounced reduction of network spike activity and abolished LTP induced by high-frequency stimulation of Schaffer's collaterals in CA1 pyramidal neurons of hippocampal slices. Antioxidant Trolox,the hydrosoluble vitamin E analogue, prevented neurotoxic effect and alterations of network activity under Cu2+ exposure, but didn't change the impairment of LTP in Cu2+-exposured hippocampal slices. We hypothesized that spontaneous network neuronal activity probably is one of the potential targets of Cu2 +-induced neurotoxicity, in which free radicals can be involved. At the same time, it may be suggested that Cu2+-induced alterations of long-lasting trace processes (like LTP) are not mediated by oxidative damage. [ DOI ]

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