Analysis of Content of 2-Oxoacids in Rat Brain Extracts Using High-Performance Liquid Chromatography

Tashlitsky, V.N.; Artiukhov, A.V.; Fedorova, N.V.; Sukonnikov, M.A.; Ksenofontov, A.L.; Bunik, V.I.; Baratova, L.A.

Авторы: Tashlitsky Vadim N., Artiukhov Artem V., Fedorova Natalia V., Sukonnikov Maxim A., Ksenofontov Alexander L., Bunik Victoria I., Baratova Ludmila A.
Журнал: Biochemistry (Moscow)
Том: 87
Номер: 4
Год издания: 2022
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 356
Последняя страница: 365
Оригинальные версии: Определение 2‑оксокислот в экстрактах мозга крыс с помощью высокоэффективной жидкостной хроматографии
DOI: 10.1134/s0006297922040058
Аннотация: 2-Oxoacids are involved in a number of important metabolic processes and can be used as biomarkers in some human diseases. A new optimized method for quantification of 2,4-dinitrophenylhydrazine derivatives of 2-oxoacids using high-performance liquid chromatography was developed based on available techniques for quantification of 2-oxoacids in mammalian brain. The use of the 2,4-dinitrophenylhydrazine derivatives of 2-oxoacids was shown to be more advantageous in comparison with the previously used phenylhydrazine derivatives, due to a high chemical stability of the former. Here, we determined the concentrations of pyruvate, glyoxylate, 2-oxoglutarate, 2-oxomalonate, and 4-methylthio-2-oxobutyrate in the methanol/acetic acid extracts of the rat brain using the developed method, as well discussed the procedures for the sample preparation in analysis of mammalian brain extracts. The validation parameters of the method demonstrated that the quantification limits for each of the analyzed of 2-oxoacids was 2 nmol/mg tissue. The developed method facilitates identification of subtle changes in the tissue and cellular content of 2-oxoacids as (patho)physiological biomarkers of metabolism in mammalian tissues.
Добавил в систему: Ксенофонтов Александр Леонидович

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Analysis of Content of 2-Oxoacids in Rat Brain Extracts Using High-Performance Liquid Chromatographyстатья

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