Immobilization of a Mixed Culture of Oxygenic Phototrophic Microorganisms on a Chitosan-Based Sorbent for Nutrient Bioremovalстатья
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Дата последнего поиска статьи во внешних источниках: 1 апреля 2022 г.
Аннотация:The immobilization of cells of a mixed culture of the microalgae (MA) Micractinium sp. NAMSUA-19 and cyanobacteria (CB) Synechococcus sp. 1Dp66E-1 on a chitosan-based polymer was studied. A polycationicsorbent based on a natural chitosan polymer with a molecular weight of 600 kDa was obtained fromthe crosslinking of chitosan with glutaraldehyde via cryopolymerization. It has a high affinity for the surfacestructures of oxygenic phototrophic microorganisms (OPMs) and enables strong cellular attachment to thesorbent surface. The study of the kinetics and evaluation of the effectiveness of mixed-culture immobilizationshowed a high sorption capacity of the chitosan sorbent. During the first hour of cultivation, the immobilizationefficiency was on average 40–52%, and, almost all cells were immobilized after 48 h. The highlyporous, nontoxic, and biodegradable sorbent provided steady cellular attachment for 7 days of cultivation anddid not affect the growth of immobilized mixed culture, either on the surface or in the inner layers of the polymer.The study of mixed-culture immobilization via scanning electron microscopy showed that CB and MAcells are tightly attached to the surface of the chitosan sorbent. This is followed by the formation of strands ofthe extracellular polymer matrix and a biofilm consisting of cells of the mixed culture of MA and CB and heterotrophicbacteria associated with Micractinium sp. Immobilization on a chitosan sorbent contributes to anincrease in the bioremoval of nitrates and phosphates by the tested mixed culture.