A Microtiter-Plate Chemiluminescence Method for the Determination of microRNAs-141 Based on the Application of Catalytic Hairpin Assembly and a Streptavidin–Polyperoxidase Conjugate

Авторы: Bodulev O.L., Sakharov I.Yu
Журнал: Zhurnal Analiticheskoi Khimii
Том: 77
Номер: 4
Год издания: 2022
Первая страница: 450
Последняя страница: 457
DOI: 10.1134/S1061934822040050
Аннотация: Currently, microRNA attract increased attention of researchers as promising markers of varioushuman diseases. This work presents a heterogeneous chemiluminescence method for the quantitative determinationof tumor marker microRNA-141, based on an enzyme-free amplification reaction, given the namecatalytic hairpin assembly (CHA). To additionally enhance the sensitivity of the method, a detection system with the double amplification of the signal, including the use of a streptavidin–horseradish polyperoxidase conjugate and an enhanced chemiluminescence reaction, was used. Because of the use of the triple amplificationstrategy, the method is characterized by the high sensitivity and the low limit of detection. It was found that this method for the determination of microRNA-141 is characterized by high specificity through the use of CHA. The specified features of the method suggest its successful application to clinical diagnostics.
Добавил в систему: Сахаров Иван Юрьевич

№	Имя	Описание	Имя файла	Размер	Добавлен
1.	Первая страница		PrintSceen_Eng.docx	244,4 КБ	3 октября 2022 [sakharov]
2.	PrintScreen.docx		PrintScreen.docx	271,9 КБ	5 апреля 2022 [sakharov]

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