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Genetic analysis of DNA excreted in urine: A new approach for detecting specific genomic DNA sequences from cells dying in an organismстатья
Дата последнего поиска статьи во внешних источниках: 8 апреля 2022 г.
- Авторы: Botezatu, (Botezatu I., Serdyuk I), (Serdyuk O., Potapova O), (Potapova G., Shelepov G), (Shelepov V., Alechina V), (Alechina R., Molyaka R), (Molyaka Y., Anan'ev Y), (Anan'ev V., Bazin V), (Bazin I., Garin I), (Garin A., Narimanov A), (Narimanov M., M
- Журнал: Open Clinical Chemistry Journal
- Том: 46
- Год издания: 2000
- Издательство: Bentham Science Publishers
- Местоположение издательства: Netherlands
- Первая страница: 1078
- Последняя страница: 1084
- Аннотация: Genetic analysis of DNA excreted in urine: A new approach for detecting specific genomic DNA sequences from cells dying in an organism By Botezatu, I (Botezatu, I) Serdyuk, O (Serdyuk, O) Potapova, G (Potapova, G) Shelepov, V (Shelepov, V) Alechina, R (Alechina, R) Molyaka, Y (Molyaka, Y) Anan'ev, V (Anan'ev, V) Bazin, I (Bazin, I) Garin, A (Garin, A) Narimanov, M (Narimanov, M) ...More View Web of Science ResearcherID and ORCID (provided by Clarivate) CLINICAL CHEMISTRY Volume46 Issue8 Page1078-1084 Part1 PublishedAUG 2000 Indexed2000-08-01 Document TypeArticle Abstract Background: Cell-free DNA from dying cells recently has been discovered in human blood plasma. In experiments performed on animals and humans, we examined whether this cell-free DNA can cross the kidney barrier and be used as a diagnostic tool. Methods: Mice received subcutaneous injections of either human Raji cells or purified F-32-labeled DNA. DNA was isolated from urine and analyzed by measurement of radioactivity, agarose gel electrophoresis, and PCR. In humans, the permeability of the kidney barrier to polymeric DNA was assessed by detection in urine of sequences that were different from an organism bulk nuclear DNA. Results: In the experiments on laboratory animals, we found that similar to 0.06% of injected DNA was excreted into urine within 3 days in a polymeric form and that human-specific Alu sequences that passed through the kidneys could be amplified by PCR. In humans, male-specific sequences could be detected in the urine of females who had been transfused with male blood as well as in DNA isolated from urine of women pregnant with male fetuses. K-ras mutations were detected in the urine of patients with colon adenocarcinomas and pancreatic carcinomas. Conclusions: The data suggest that the kidney barrier in rodents and humans is permeable to DNA molecules large enough to be analyzed by standard genetic methodologies. (C) 2000 American Association for Clinical Chemistry. Keywords Keywords PlusLUNG-CANCER PATIENTSFETAL RHD STATUSMATERNAL PLASMAMICROSATELLITE ALTERATIONSQUANTITATIVE-ANALYSISPRENATAL-DIAGNOSISCOLORECTAL-CANCERTUMOR DNASERUM DNAMARKER Addresses DIAGEN Corp, Richmond, CA USA Russian Acad Med Sci, Canc Res Ctr, Inst Carcinogenesis, Moscow 115478, Russia Russian Acad Med Sci, Canc Res Ctr, Inst Clin Oncol, Moscow 115478, Russia Ctr Med Genet, Moscow 115478, Russia Categories/Classification Research AreasMedical Laboratory Technology
- Добавил в систему: Базин Игорь Сергеевич