System of Markers Based on the Methylation of a Group of Proapoptotic Genes in Combination with MicroRNA in the Diagnosis of Breast Cancerстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 3 февраля 2022 г.
Аннотация:Systems of markers for the diagnosis of breast cancer based on DNA methylation of a group of suppressor protein-coding genes, hypermethylated microRNA genes, and their combinations were compiled. On a representative sample of 70 paired breast cancer specimens (tumor/normal), MS-PCR analysis revealed a significant increase in the methylation frequency of 5 protein-coding genes: RASSF1A suppressor and apoptosis genes APAF1 , BAX , BIM/BCL2L11 , and DAPK1 (34-61% vs . 4-24%) and 6 microRNA genes: MIRG124G1 , MIRG125bG1 , MIRG129G2 , MIRG148a , MIRG34b/c , and MIRG9G3 (36-76% vs . 6-27%). ROC-analysis showed that a combination of 4 genes ( APAF1 , BAX , BIM/BCL2L11 , and DAPK1 ) and MIRG125bG1 gene constitute a highly efficient 5-marker system with 100% specificity and sensitivity of 94-96% at AUC=0.98-0.97, suitable also for patients with stage I and II breast cancer. Detection of methylation of at least one gene in this system in biopsy or postoperative material is sufficient to refer the sample to breast cancer.