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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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New transgenic mice with overexpression of human interleukin‐6 in myeloid cellsтезисы доклада
- Авторы: Medvedovskaya A.D., Gorshkova E.A., Gubernatorova E.O., Zvartsev R.V., Ustyugov A.A., Drutskaya M.S., Nedospasov S.A.
- Сборник: European Journal of Immunology (2021 г.)
- Серия: Special Issue
- Том: 51
- Тезисы
- Год издания: 2021
- Первая страница: 220
- Номер статьи: P‐0321
- Аннотация: L‐6 is a pleiotropic cytokine involved in regulation of inflammation, metabolism, tissue homeostasis and repair. Recently, IL‐6 was identified as one of the prime candidates for mediating inflammation in COVID‐19 patients and as a key player in the cytokine storm, a condition caused by extensive activation of the immune system. However, long‐term consequences of systemic IL‐6 elevation were not yet systematically studied. Therefore, mice with cell type‐specific overexpression of IL‐6 provide a useful model for evaluating the effects of chronic overabundance of this cytokine. Using loxP‐Cre technology, we generated mice with transgenic overexpression of human IL‐6 and reporter fluorescent protein EGFP in cells of myeloid lineage. These mice (hIL‐6 Tg LysM‐cre) showed retardation in the postnatal growth and reduced lifespan as compared to littermate control mice. Elevated serum level of human IL‐6 was accompanied by an increase in GM‐CSF, IL‐17, IL‐15 and CXCL10. Transgenic hIL‐6 Tg LysM‐cre mice developed hyperinflammation with extreme splenomegaly, lymph node hyperplasia, and neutrophilia of vital organs at the age of 8‐10 weeks. In addition, hIL‐6 Tg LysM‐cre mice demonstrated erythrocytopenia in the bone marrow and showed blood clotting perturbations, thus, displaying disorders characteristic of acute cytokine release syndrome with high levels of IL‐6, as in case of severe COVID‐19. New transgenic mice with regulated overexpression of human IL‐6 in specific cell type should be useful for studying and mechanistic understanding of severe inflammatory syndromes. They can also be used as preclinical models for testing novel therapeutic approaches.
- Добавил в систему: Губернаторова Екатерина Олеговна