Effect of acoustic pulses and EHF radiation on multipotent marrow stromal cell in tissue engineering constructionsстатья

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Дата последнего поиска статьи во внешних источниках: 11 апреля 2017 г.

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[1] Effect of acoustic pulses and ehf radiation on multipotent marrow stromal cell in tissue engineering constructions / R. K. Chailakhyan, V. I. Yusupov, Y. F. Gorskaya et al. // Journal of innovative optical health sciences. — 2016. — Vol. 10, no. 1. — P. 1650036–1–1650036–9. In this paper, we studied the e®ects of physical factors, such as, acoustic pulses of laser-induced hydrodynamics (ALIH) and extremely-high frequencies (EHF) radiation, on the formation of heterotopic bone marrow organs. A suspension of precipitated bone marrow cells from CBA mice were exposed to ALIH pulses and EHF radiation separately and in their combination tissue engineering constructs, presenting gelatin sponges 2 by 2 by 2 mm in size containing 107 nucleated bone marrow cells, were exposed to physical factors and were implanted under the renal capsules of syngeneic mice. The newly formed hematopoietic organs were examined in three and ¯ve months later after treatment. The ¯ve months old transplants were bigger in size than the three months old transplants. The number of hematopoietic cells in the rest of the groups increased during this period by a factor from 3 to 10, the increase being as high as 20-fold in the ALIHþEHF group. Maximal concentration of multipotent stromal cells (MSCs) was in the EHFþALIH, and minimal concentration was in the ALIHþEHF. The accumulation rate of bone capsule weight was highest for the transplants of EHFþALIH and ALIH-sponge groups during the ¯rst three months. These data showed that the combined impact of the EHFþALIH on MSCs is the most e®ective for the formation of bone marrow transplantation. [ DOI ]

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