Early alteration of nucleocytoplasmic traffic induced by some RNA virusesстатья

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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.

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[1] Early alteration of nucleocytoplasmic traffic induced by some rna viruses / G. A. Belov, A. G. Evstafieva, Y. P. Rubtsov et al. // Virology. — 2000. — Vol. 275, no. 2. — P. 244–248. A HeLa cell line expressing the green fluorescent protein fused to the SV40 T-antigen nuclear localization signal (EGFP-NLS) was established. Fluorescence in these cells was confined to the nuclei. After poliovirus infection, cytoplasmic fluorescence in a proportion of cells could be detected by 1 h postinfection (p.i.) and in virtually all of the fluorescent cells by 2 h p.i. The relocation could be prevented by cycloheximide but not by inhibition of poliovirus replication by guanidine HCl. Nuclear exit of a protein composed of three copies of GFP fused to the NLS also occurred upon poliovirus infection. A similar redistribution of EGFP-NLS took place upon infection with coxsakievirus B3 and, to a lesser extent, with vesicular stomatitis virus. The EGFP-NLS efflux was not due to the loss of NLS. Thus, some positive-strand and negative-strand RNA viruses trigger a rapid nonspecific relocation of nuclear proteins. (C) 2000 Academic Press. [ DOI ]

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